Basic requirements for microbiology laboratory

(1) Preparation room

The preparation room is used for preparing medium and sample processing. There are reagent cabinets, counters for storing utensils or materials, experimental platforms, electric furnaces, refrigerators and drains, power supplies, etc.

(2) cleaning room

Cleaning room for washing utensils, etc. Because used utensils have been contaminated with microorganisms, sometimes pathogenic microorganisms can also be present. Therefore, if conditions permit, it is best to set up a cleaning room. Indoor should be equipped with heaters, cooking POTS, washing utensils with POTS, buckets, etc., but also should have a variety of bottle brushes, detergent powder, soap, washing powder, etc.

(3) Sterilization room

Sterilization room is mainly used for the sterilization of culture medium and various instruments to kill pathogenic microorganism classification list bacteria, indoor should be equipped with high pressure steam sterilizer, oven and other sterilization equipment and facilities.

(4) aseptic room

Asepsis room, also known as inoculation room, is a special laboratory for aseptic operation such as systematic inoculation and purification of bacteria. In microbial matters, the inoculation and transplantation of strains is a major operation, which is characterized by the inclusion of pure strains to prevent the contamination of miscellaneous bacteria. In the air of the general environment, due to the existence of a lot of dust and miscellaneous bacteria, it is easy to cause pollution, which interferes greatly with the inoculation.

(5) Constant temperature culture room

1. Setting of the culture room

(1) The culture room should be inside and outside two, cabinet room is the culture room, the outer room is the buffer room. The room volume should not be large, in order to facilitate air sterilization, the cabinet room area is about 3.2×4.4 = 14m2, the outer room area is about 3.2×1.8 = 6m2, and the height is about 2.5m, which should be Tianhua board.

(2) The upper part of the wall separating the cabinet room and the outer room should be equipped with a vent with air filter device.

(3) In order to meet the needs of microorganisms for temperature, constant temperature and humidity machines should be installed.

(4) Both inside and outside the room should be installed in the center of the room UV lamp for sterilization.

2. Cultivate indoor equipment and appliances

(1) Cabinet room is usually equipped with culture rack and bottle shaking machine (shaking bed). Commonly used shake bottle machine is rotary, reciprocating two.

(2) The outer room should have special clothes, shoes, hats, masks, hand-held sprayers, 5% coal acid solution, 70% alcohol cotton balls, etc.

3. Sterilization and disinfection of culture room

Sterilization and disinfection measures of aseptic room.

A small range of culture can not be used in a constant temperature incubator, but held in a constant temperature incubator.

(6) General laboratories

A place where observation, counting, and physiological and biochemical determination of microorganisms are performed. The furnishings of the room vary greatly because of the difference in emphasis. Generally equipped with laboratory bench, micromicrobiology bulletin, cabinet and stool. The experimental platform is required to be smooth and smooth, and the experimental cabinet should be enough to accommodate the same commonly used appliances and drugs.

(7) Other requirements of the laboratory

The capacity, layout and performance of water, electricity and gas should meet the needs of the laboratory.